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  • BD 559763 PE Annexin V Apoptosis Detection Kit
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  • BD 559763 PE Annexin V Apoptosis Detection Kit 细胞凋亡双染试剂盒,复申生物长期现货销售!凡购买BD BD 规格100T 556547 FITC Annexin V Apoptosis Detection Kit I 细胞凋亡双染试剂盒
  • 400-998-5068/021-33565660
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BD 559763 PE Annexin V Apoptosis Detection Kit I 细胞凋亡双染试剂盒

DESCRIPTION

 

BD 559763 PE Annexin V Apoptosis Detection Kit I 细胞凋亡双染试剂盒

Apoptosis is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis. The apoptotic program is characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment, condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. Loss of plasma membrane is one of the earliest features. In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, thereby exposing PS to the external cellular environment. Annexin V is a 35-36 kDa Ca2+ dependent phospholipid-binding protein that has a high affinity for PS, and binds to cells with exposed PS. Annexin V may be conjugated to fluorochromes including Phycoerythrin (PE). This format retains its high affinity for PS and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis. Since externalization of PS occurs in the earlier stages of apoptosis, PE Annexin V staining can identify apoptosis at an earlier stage than assays based on nuclear changes such as DNA fragmentation.

 

PE Annexin V staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic processes. Therefore, staining with PE Annexin V is typically used in conjunction with a vital dye such as 7-Amino-Actinomycin (7-AAD) to allow the investigator to identify early apoptotic cells (7-AAD negative, PE Annexin V positive). Viable cells with intact membranes exclude 7-AAD, wheras the membranes of dead and damaged cells are permeable to 7-AAD. For example, cells that are considered viable are PE Annexin V and 7-AAD negative; cells that are in early apoptosis are PE Annexin V positive and 7-AAD negative; and cells that are in late apoptosis or already dead are are both PE Annexin V and 7-AAD positive. This assay does not distinguish between cells that have undergone apoptotic death versus those that have died as a result of a necrotic pathway because in either case, the dead cells will stain with both PE Annexin V and 7-AAD. However, when apoptosis is measured over time, cells can be often tracked from PE Annexin V and 7-AAD negative (viable, or no measurable apoptosis), to PE Annexin V positive and 7-AAD negative (early apoptosis, membrane integrity is present) and finally to PE Annexin V and 7-AAD positive (end stage apoptosis and death). The movement of cells through these three stages suggests apoptosis. In contrast, a single observation indicating that cells are both PE Annexin V and 7-AAD positive, in of itself, reveals less information about the process by which the cells underwent their demise.

 

 

COMPONENTS

Part No.DescriptionSize
51-66121E10X Annexin V Binding Buffer50 mL (1 ea)
51-65875XPE Annexin V0.5 mL (1 ea)
51-68981E7-AAD2 mL (1 ea)

RESOURCES & TOOLS

  • Download MSDS
  • Download TDS

PREPARATION AND STORAGE

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

PRODUCT NOTICES

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences。。com/pharmingen/protocols for technical protocols.
  5. BD 559763 PE Annexin V Apoptosis Detection Kit I 细胞凋亡双染试剂盒 检测凋亡细胞膜的变化

  6. 磷脂酰丝氨酸检测:Annexin V试剂盒
    检测方法:FACS,荧光或光学显微镜
    正常细胞中,磷脂酰丝氨酸只分布在细胞膜脂质双层的内侧,细胞发生凋亡早期,膜磷脂酰丝氨酸(PS)由脂膜内侧翻向外侧。Annexin V是一种磷脂结合蛋白,与磷脂酰丝氨酸有高度亲和力,它通过细胞外侧暴露的磷脂酰丝氨酸与凋亡早期细胞的胞膜结合。因此Annexin V是检测细胞早期凋亡的灵敏指标。将Annexin-V与PI匹配使用,可以将凋亡早晚期的细胞以及死细胞区分开来。
  7.  

    品牌

    品名

    货号

    规格

    Roche

    Annexin-V-FLUOS

    11828681001

    500 μl (250 tests)

    Annexin-V-Alexa 568

    03703126001

    500 μl (250 tests)

    Annexin-V-Biotin

    11828690001

    500 μl (250 tests)

    Annexin-V-FLUOS Staining Kit

    11858777001

    1 kit (50 tests)

    11988549001

    1 kit (250 tests)

    BD

    Annexin V : FITC Apoptosis Detection Kit I

    556547

    100 tests

    Annexin V : FITC Apoptosis Detection Kit I

    556570

    100 tests

    Annexin V : FITC Fluorescence Microscopy Kit

    550911

    25 Slides

    Annexin V : PE Apoptosis Detection Kit I

    559763

    100 tests

    检测凋亡细胞线粒体的变化

  8. 线粒体通路:
    凋亡早期改变之一是线粒体外膜通透性增加。线粒体外膜透化的实现涉及到许多蛋白的参与,特别是Bcl-2家族成员。促凋亡的Bcl-2家族成员Bax及Bak有助于线粒体外膜透化,进而引起caspase9的活化;而抗凋亡的Bcl-2/Bcl-XL起到抑制凋亡的作用。
  9.  

    货号

    品名

    规格

    612742

    Bcl-2 Related Sampler Kit

    10ul each

    612741

    Apoptosis I Sampler Kit

    10ul each

    检测凋亡细胞核的变化

  10. DNA ladder:
    细胞凋亡时主要的生化特征是其染色质发生浓缩,染色质DNA在核小体单位之间的连接处断裂,形成50~300kbp长的DNA大片段,或180~200bp整数倍的寡核苷酸片段,在凝胶电泳上表现为梯形电泳图谱(DNA ladder)。
  11.  

    货号

    品名

    规格

    Roche

    11835246001

    Apoptotic DNA-Ladder Kit

    1 Kit (20Test)

    11774425001

    Cell Death Detection ELISA

    1 kit (96 tests)

  12. TUNEL(荧光,POD,AP,TMR)试剂盒
    细胞凋亡中, 染色体DNA 双链断裂或单链断裂而产生大量的粘性3‘-OH 末端,可在脱氧核糖核苷酸末端转移酶(TdT)的作用下,将脱氧核糖核苷酸和荧光素、过氧化物酶、碱性磷酸酶或生物素形成的衍生物标记到DNA的3’-末端,从而进行凋亡细胞的检测,这类方法称为脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(terminal-deoxynucleotidyl transferase mediated nick end labeling, TUNEL)。由于正常的或正在增殖的细胞几乎没有DNA 的断裂,因而没有3‘-OH 形成,很少能够被染色。
  13.  

    品名

    检测样本

    规格

    货号

    Roche

    In Situ Cell Death Detection Kit,AP

    细胞涂片,贴壁细胞,细胞甩片,冷冻货固定组织切片

    50T

    11684809910

    In Situ Cell Death Detection Kit,POD

    50T

    11684817910

    In Situ Cell Death Detection Kit,Fluorescein

    贴壁细胞,悬浮细胞,细胞涂片,冷冻或石蜡切片

    50T

    11684795910

    In Situ Cell Death Detection Kit, TMR red

    50T

    12156792910

    BD

    Apoptosis Detection Kit (APO-Direct)

     

    50 tests

    556381

    Apoptosis Detection Kit (APO-BRDU)

     

    60 tests

    556405

    检测凋亡细胞胞质的变化

  14. Caspase3
    Caspase-3正常以酶原的形式存在于胞浆中,在凋亡的早期阶段被激活,活化的Caspase-3由两个大亚基(17KD)和两个小亚基(12KD)组成,裂解相应的胞浆胞核底物,最终导致细胞凋亡。但在细胞凋亡的晚期和死亡细胞,caspase-3的活性明显下降。
  15.  

    品牌

    品名

    货号

    规格

    BD

    Caspase-3, Active Form, mAb Apoptosis Kit: FITC (CPP32)

    550480

    100 tests

    Caspase-3, Active Form, Human ELISA Set

    550930

    5 plates

    Roche

     

    检测对象

    检测方法

    检测样品

    品名

    规格

    货号

    Caspase对角蛋白18的裂解作用

    Western blot,流式细胞仪,荧光显微镜或光学显微镜(单细胞水平检测)

    贴壁细胞,组织样本(福尔马林/石蜡包埋切片,恒冷箱切片)

    M30 CytoDEATH

    100 μl (250 tests)

    12140349001

    M30 CytoDEATH, Fluorescein

    100 μl (250 tests)

    12156857001

    Caspase 3活性

    荧光ELISA

    细胞裂解产物,重组Caspase 3

    Caspase 3 Activity Assay

    96 tests

    12012952001

    Caspase

    荧光ELISA

    细胞裂解产物,重组Caspase

    Homogeneous Caspases Assay, fluorimetric

    100 tests on 96-well plates, 400 tests on 384-well plates

    03005372001

    Caspase对PARP的裂解作用

    Western blot,免疫沉降,免疫组织化学

    细胞粗提物

    Anti-Poly( ADP-Ribose) Polymerase (PARP)

    100 μl (50 blots)

    11835238001

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