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  • Roche地高辛标记和检测试剂盒II现货促销
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  • Roche地高辛标记和检测试剂盒II现货促销,Roche-地高辛标记检测试剂盒I,II型 现货促销,复申生物常年现货供应,下单既有好礼相送
  • 400-998-5068/021-33565660
产品详细介绍

概述

别名: 

Roche:  DIG-High Prime DNA Labeling and Detection Starter Kit II高效DNA地高辛标记和检测试剂盒II(化学发光)

该试剂盒比普通DIG随机引物法标记效率高,采用CSPO化学发光检测。灵敏度更高,一个kit可用于12次标记反应和24次检测。

反应组成;5×高效Dig标记复合物;未标记对照DNA,PBR328;DNA稀释液;抗-Dig-碱性磷酸酶连接物;CSPD;10×封闭液;Dig定量试纸条;Dig对照试纸条;NBT/BCIP储存液。 -20℃保存。

Related Categories12 Principles Aligned Products, Chemical Synthesis,Greener Alternative Products, Life Sciences
usage  sufficient for 12 labeling reactions (10 ng to 3 μg per assay)
 sufficient for 24 blots (blots of 100 cm2)
mfr. no.  Roche
greener alternative product characteristics  Designing Safer Chemicals
Learn more about the Principles ofGreen Chemistry.
shipped in  dry ice
storage temp.  −20°C

研究领域: 

脱氧核糖核酸

其他

详述

Application

DIG-High Prime is used for the highly efficient random-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile. DIG-labeled probes are generated at high yield within one hour or after overnight incubation. DIG-High Prime labeled DNA probes are used in a variety of hybridization techniques: • Southern blots
• Northern blots
• Dot blots
• Colony and plaque hybridizations
• For all types of filter hybridization
• For single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley, and wheat

General description

Download the CofA, SDS and IFU specific to your product lot and format

Convenient kit for random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

1 kit containing 7 components.

Principle

The DIG High Prime DNA Labeling and Detection Starter Kit II uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent chemiluminescence detection by enzyme immunoassay. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves solely as a template for the synthesis of labeled DNA, and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10 ng) with this method.The complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin present in the reaction, are incorporated into the newly synthesized complementary DNA strand.

Roche地高辛标记及检测相关试剂盒

名称

编号

产地

规格

价格¥

DIG DNA Labeling and Detection Kit

地高辛DNA标记和检测试剂盒

11093657910 

Roche

Kit

8732

DIG-11-dUTP随机引物法标记DNA, 碱性磷酸酶(AP)生色反应检测, 标记探针用于Southern、Northern和斑点杂交以及菌落和噬菌体筛选。组成: 未标记对照DNA1; 未标记对照DNA2; DNA稀释液; DIG-标记对照DNA; 六核苷酸混合物; Klenow 2u/μl; 封闭液; BCIP/NBT; 10×dNTP标记混合物; 抗-地高辛-碱性磷酸酶复合物。一个Kit可用于25次标记反应和50次检测(100cm2膜), -20℃保存。

 

DIG-High Prime DNA Labeling and

Detection Starter Kit I

高效DNA地高辛标记和检测试剂盒I

11745832910      

Roche

Kit

5457

比普通DIG随机引物法标记效率高, 操作简单, 可用于Southern、Northern和斑点杂交以及菌落和噬菌体筛选。一个Kit可用于12次标记反应和24次检测反应。组成: 5×DIG-高效标记试剂; 未标记对照DNA, PBR328; DNA稀释液; 抗-DIG-碱性磷酸酶复合物; NBT/BCIP储存液; 10×封闭液; DIG定量复试纸条; DIG对照试纸条, -20℃保存。

DIG-High Prime DNA Labeling and

Detection Starter Kit II

高效DNA地高辛标记和检测试剂盒II

11585614910

Roche

Kit

5598

比普通DIG随机引物法标记效率高, 采用CSPD化学发光检测灵敏度更高。一个Kit可用于12次标记反应和24次检测反应。组成: 5×高效DIG标记复合物; 未标记对照DNA, PBR328; DNA稀释液; 抗-DIG-碱性磷酸酶连接物; CSPD; 10×封闭液; DIG定量试纸条; DIG对照试纸条, NBT/BCIP储存液; -20℃保存。

 

DIG DNA Labeling Kit

地高辛DNA标记试剂盒

11175033910

Roche

40T

7753

随机引物扩增法以DIG-11-dUTP标记DNA; DIG标记探针可用于各种膜杂交。

DIG Nucleic acid Detection Kit

地高辛核酸检测试剂盒

11175041910

Roche

Kit

5165

NBT/BCIP显色法检测, 灵敏度可达0.1pg同源DNA; 可检测40张100cm2杂交膜。-20℃保存。

DIG Oligonucleotide 3’-End Labeling Kit

寡核苷酸3’末端地高辛标记试剂盒

3353575910

Roche

25T

7432

通过末端转移酶以DIG-11-ddUTP标记14-100bp的寡核苷酸探针3’端用于各种膜杂交和原位杂交。每个反应可标记100pmol的寡聚核苷酸。

DIG Oligonucleotide Tailing Kit

寡核苷酸加尾地高辛标记试剂盒

3353583910

Roche

25 T

6266

通过末端转移酶以DIG-11-ddUTP在寡核苷酸探针3’端加一平均长度50bp(10-100bp)的寡核苷酸, 用于DNA/RNA膜杂交和原位杂交。每个反应可标记100pmol的寡聚核苷酸。

 

DIG RNA Labeling Kit (SP6/T7)

地高辛RNA标记试剂盒(SP6/T7)

11175025910

Roche

2´10T

7592

由SP6或T7 RNA聚合酶通过转录反应过程标记RNA, 1mg模板可产生约10mg标记RNA用于杂交。-20℃保存。组成: pSPT18 DNA, pSPT19 DNA, 对照DNA1, 对照DNA2, DIG标记对照RNA, NTR标记混合物, 转录缓冲液, RNase抑制剂, 无RNase的DNase I, SP6 RNA聚合酶, T7 RNA聚合酶。

PCR DIG Probe Synthesis Kit

PCR地高辛探针合成试剂盒

11636090910

Roche

25T

5598

标记的探针可在Southern杂交后从5-10mg的人基因组DNA检测出单拷贝基因, 是从少量DNA模板制备大量探针的最有效、最经济的方法。

DIG Northern Starter Kit

12039672910

Roche

10T

5052

包含标记DIG RNA及进行Northern印迹的所有必需试剂, 配合DIG Wash and Block Buffer Set方便进行Northern印迹; 能作10个标记反应及10次检测(100mm2膜)。特别适合初用者使用; 标记混合物经最优化, 配合提供的化学发光底物CDP-Star, 能产生最高的灵敏度。

DIG Luminescent Detection Kit

地高辛发光检测试剂盒

 11363514910

Roche

Kit

5627

CSPD化学发光法检测, 灵敏度可达0.03pg同源DNA; 可检测50张100cm2杂交膜。

DIG High Prime

高效地高辛DNA标记试剂

11585606910

Roche

40T

7838

标记DNA探针, 只需一步操作, 标记效率较通常随机引物法高3倍。40 T

储存与安全

储存: 

储存与安全

储存: 

−20°C

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